Press:

Nanoligent desarrolla nuevo fármaco anticanceroso. La Vanguardia

Nanoligent nova spin-off de la UAB i Hospital de Sant pau

 

Mechanism of action:

Ugutz Unzueta, María Virtudes Céspedes, Neus Ferrer-Miralles, Isolda Casanova,  Juan Cedano, José Luis Corchero, Joan Domingo-Espín, Antonio Villaverde, Ramón Mangues, Esther Vázquez. 2012. Intracellular CXCR4+ cell targeting with T22-empowered protein-only nanoparticles. International Journal of Nanomedicine 7: 4533 – 4544.  https://doi.org/10.2147/IJN.S34450

Laura Sánchez-García, Naroa Serna, Patricia Álamo, Rita Sala, María V Céspedes, Mònica Roldan, Alejandro Sánchez-Chardi, Ugutz Unzueta, Isolda Casanova, Ramon Mangues, Esther Vázquez, Antonio Villaverde. 2018. Self-assembling toxin-based nanoparticles as self-delivered antitumoral drugs. Journal of Controlled Release, 274: 81-92. https://doi.org/10.1016/j.jconrel.2018.01.031

Aïda Falgàs, Victor Pallarès, Ugutz Unzueta, María Virtudes Céspedes, Irene Arroyo-Solera, María José Moreno, Alberto Gallardo, María Antonia Mangues, Jorge Sierra, Antonio Villaverde, Esther Vázquez, Ramon Mangues, and Isolda Casanova. 2020. A CXCR4-targeted nanocarrier achieves highly selective tumor uptake in diffuse large B-cell lymphoma mouse models. Haematologica. 2020. 105: 741-753. https://doi.org/10.3324/haematol.2018.211490

Vehicle design and properties:

Céspedes MV, Unzueta U, Tatkiewicz W, Sánchez-Chardi A, Conchillo-Solé O, Alamo P, Xu Z, Casanova I, Corchero JL, Pesarrodona M, Cedano J, Daura X, Ratera I, Veciana J, Ferrer-Miralles N, Vazquez E, Villaverde A, Mangues R. 2014. In Vivo Architectonic Stability of Fully de Novo Designed Protein-Only Nanoparticles. ACS Nano. 8:4166-4176. https://doi.org/10.1021/nn4055732

Unzueta U, Céspedes MV, Ferrer-Miralles N, Vazquez E, Mangues R., Villaverde A. 2015. Towards Protein-Based Viral Mimetics for Cancer Therapies. Trends in Biotechnology, 33: 253-258. https://doi.org/10.1016/j.tibtech.2015.02.007

Naroa Serna, Laura Sánchez-García, Ugutz Unzueta, Raquel Díaz, Esther Vázquez, Ramón Mangues and Antonio Villaverde. 2018. Protein-based therapeutic killing for cancer therapies. Trends in Biotechnology, 36: 318-335 https://doi.org/10.1016/j.tibtech.2017.11.007

Hèctor López-Laguna, Julieta Sánchez, Ugutz Unzueta, Ramón Mangues, Esther Vázquez and Antonio Villaverde. 2020. Divalent cations: A molecular glue for protein materials. Trends in Biochemical Sciences 45: 992-1003. ://doi.org/10.1016/j.tibs.2020.08.003

Patricia Alamo, Juan Cedano, Oscar Conchillo-Sole, Olivia Cano-Garrido, “Lorena Alba-Castellon, Naroa Serna, Anna Aviño, Luis Miguel Carrasco-Diaz, Alejandro Sanchez-chardi, Carlos Martinez-Torro, Alberto Gallardo, Montserrat Cano, Ramon Eritja, Antonio Villaverde, Ramon Mangues, Esther Vazquez, Ugutz Unzueta. 2021. Rational engineering of a human GFP-like protein scaffold for humanized targeted nanomedicines. Acta Biomaterialia 130, 211-222 https://doi.org/10.1016/j.actbio.2021.06.001

López-Laguna, Hèctor; Sánchez, Julieta; Carratalá, José Vicente; Rojas-Peña, Mauricio; Sánchez-García, Laura; Parladé, Eloi; Sánchez-Chardi, Alejandro; Voltà-Durán, Eric; Serna, Naroa; Cano-Garrido, Olivia; Flores, Sandra; Ferrer-Miralles, Neus; Nolan, Verónica; de Marco, Ario; Roher, Nerea; Unzueta, Ugutz; Vazquez, Esther; Villaverde, Antonio. 2021. Biofabrication of functional protein nanoparticles through simple His-tag engineering. ACS Sustainable Chemistry & Engineering, 9, 12341-12354. https://doi.org/10.1021/acssuschemeng.1c04256

Naroa Serna, Victor Pallarès, Ugutz Unzueta, Alejandro Sánchez-Chardi, Eric Voltà-Durán, Eloi Parladé, Isolda Casanova, Aïda Falgàs, Lorena Alba-Castellón, Jorge Sierra, Antonio Villaverde, Esther Vázquez and Ramón Mangues. 2022. Engineering non-antibody human proteins as efficient scaffolds for selective, receptor-targeted drug delivery. Journal of Controlled Release, 343: 277-287. https://doi.org/10.1016/j.jconrel.2022.01.017

Efficacy on liquid tumors:

Nanotoxin

Aïda Falgàs, Victor Pallarès, Naroa Serna, Laura Sánchez-García, Jorge Sierra, Alberto Gallardo, Lorena Alba-Castellón, Patricia Álamo, Ugutz Unzueta, Antonio Villaverde, Esther Vázquez, Ramon Mangues and Isolda Casanova. 2020. Selective delivery of T22-PE24-H6 to CXCR4+ diffuse large B-cell lymphoma cells leads to wide therapeutic index in a disseminated mouse model. Theranostics. 10: 5169-5180. https://www.thno.org/v10p5169.htm

Victor Pallarès, Yáiza Núñez, Laura Sánchez-García, Aïda Falgàs, Naroa Serna, Ugutz Unzueta, Alberto Gallardo, Lorena Alba-Castellón, Patricia Álamo, Jorge Sierra, Antonio Villaverde, Esther Vázquez, Isolda Casanova, Ramon Mangues. 2021. Antineoplastic effect of a diphtheria toxin-based nanoparticle targeting acute myeloid leukemia cells overexpressing CXCR4. Journal of Controlled Release 335: 117-129. https://doi.org/10.1016/j.jconrel.2021.05.014

Yáiza Núñez, Annabel Garcia-León, Aïda Falgàs, Naroa Serna, Laura Sánchez-García, Ana Garrido, Jorge Sierra, Alberto Gallardo, Lorena Alba-Castellón, Patricia Álamo, Ugutz Unzueta, Esther Vázquez, Antonio Villaverde, Ramon Mangues, Isolda Casanova. 2023. T22-PE24-H6 nanotoxin selectively kills CXCR4-high expressing AML patient cells in vitro and potently blocks dissemination in vivo. Pharmaceutics 15, 727. https://doi.org/10.3390/pharmaceutics15030727

Nanoconjugate

Naroa Serna; Victor Pallarès; Ugutz Unzueta; Aïda Falgàs; Laura Sánchez-García; Naroa Serna; Alberto Gallardo; Gordon A. Morris; Lorena Alba-Castellón; Patricia Álamo; Jorge Sierra; Antonio Villaverde; Esther Vázquez; Isolda Casanova; Ramon Mangues. 2020. An Auristatin nanoconjugate targeting CXCR4+ leukemic cells blocks acute myeloid leukemia dissemination. Journal of Hematology & Oncology, 13, 1-19. https://doi.org/10.1186/s13045-020-00863-9

Aïda Falgàs, Victor Pallarès, Ugutz Unzueta, Yáiza Núñez, Annabel García-León, Jorge Sierra, Alberto Gallardo, Lorena Alba-Castellón, Maria Antònia Mangues, Patricia Álamo, Antonio Villaverde, Esther Vázquez, Ramon Mangues and Isolda Casanova. 2021. Specific cytotoxic effect of an auristatin nanoconjugate towards CXCR4+ diffuse large B-cell lymphoma cells. International Journal of Nanomedicine 16:1869-1888. https://doi.org/10.2147/IJN.S289733

Victor Pallarès, Ugutz Unzueta, Aïda Falgàs, Anna Aviñó, Yáiza Núñez, Annabel García-León, Laura Sánchez-García, Naroa Serna, Alberto Gallardo, Lorena Alba-Castellón, Patricia Álamo, Jorge Sierra, Lídia Cedó, Ramón Eritja, Antonio Villaverde, Esther Vázquez, Isolda Casanova, Ramon Mangues. 2022. A multivalent Ara-C-prodrug nanoconjugate achieves selective ablation of leukemic cells in an acute myeloid leukemia mouse model. Biomaterials 280, 121258. https://doi.org/10.1016/j.biomaterials.2021.121258

Efficacy on solid tumors:

Nanotoxin

Raquel Díaz, Victor Pallarès, Olivia Cano-Garrido, Naroa Serna, Laura Sánchez-Garcia, Aïda Falgàs, Mireia Pesarrodona, Ugutz Unzueta, Alejandro Sánchez-Chardi, Julieta M. Sánchez, Isolda Casanova, Dr Esther Vázquez, Ramón Mangues, Antonio Villaverde. 2018. Selective CXCR4+ cancer cell targeting and potent antineoplastic effect by a nanostructured version of recombinant ricin. Small, 14: 1800665. https://doi.org/10.1002/smll.201800665

Naroa Serna, Patricia Álamo, Prashanthi Ramesh, Daria Vinokurova, Laura Sánchez-García, Ugutz Unzueta, Alberto Gallardo, Mª Virtudes Céspedes, Esther Vázquez, Antonio Villaverde, Ramón Mangues and Jan Paul Medema. 2020. Nanostructured Toxins for the Selective Destruction of Drug Resistant Human CXCR4+ Colorectal Cancer Stem Cells. Journal of Controlled Release 320: 96–104.  https://doi.org/10.1016/j.jconrel.2020.01.019

Elisa Rioja-Blanco; Irene Arroyo-Solera; Patricia Álamo; Isolda Casanova; Alberto Gallardo; Ugutz Unzueta; Naroa Serna; Laura Sánchez-García; Miquel Quer; Antonio Villaverde; Esther Vázquez; Ramon Mangues; Lorena Alba-Castellón; Xavier León. 2022. Self-assembling protein nanocarrier for the selective delivery of cytotoxic polypeptides to CXCR4+ head and neck squamous cell carcinoma tumors. Acta Pharmaceutica Sinica B, 12: 2578-2591. https://doi.org/10.1016/j.apsb.2021.09.030

Elisa Rioja-Blanco, Irene Arroyo-Solera, Patricia Álamo, Isolda Casanova, Alberto Gallardo, Ugutz Unzueta, Naroa Serna, Laura Sánchez-García, Miquel Quer, Antonio Villaverde, Esther Vázquez, Xavier León, Lorena Alba-Castellón & Ramon Mangues. 2022. CXCR4-targeted nanotoxins induce GSDME-dependent pyroptosis in head and neck squamous cell carcinoma. Journal of Experimental & Clinical Cancer Research, 41: 49. https://doi.org/10.1186/s13046-022-02267-8

Aïda Falgàs, Annabel Garcia-León, Yáiza Núñez, Naroa Serna, Laura Sánchez-Garcia, Ugutz Unzueta, EricVoltà-Durán, Marc Aragó, Patricia Álamo, Lorena Alba-Castellón, JorgeSierra, Alberto Gallardo, Antonio Villaverde, Esther Vázquez, Ramon Mangues, Isolda Casanova. 2022. A diphtheria toxin-based nanoparticle achieves specific cytotoxic effect on CXCR4+ lymphoma cells without toxicity in immunocompromised and immunocompetent mice. Biomedicine & Pharmacotherapy, 150: 112940. https://doi.org/10.1016/j.biopha.2022.112940

Elisa Rioja-Blanco, Alberto Gallardo, Irene Arroyo-Solera, Patricia Álamo, Isolda Casanova, Ugutz Unzueta, Naroa Serna, Laura Sánchez-García, Miquel Quer, Antonio Villaverde, Esther Vázquez, Xavier León, Lorena Alba-Castellón, Ramon Mangues. 2022. A novel CXCR4-targeted diphtheria toxin nanoparticle inhibits invasion and metastatic dissemination in a head and neck squamous cell carcinoma mouse model. Pharmaceutics, 14, 887. https://doi.org/10.3390/pharmaceutics14040887

Rita Sala, Elisa Rioja-Blanco, Naroa Serna, Laura Sánchez-García, Patricia Álamo, Lorena Alba-Castellón, Isolda Casanova, Antonio López-Pousa, Ugutz Unzueta, María Virtudes Céspedes, Esther Vázquez, Antonio Villaverde & Ramon Mangues. 2022. GSDMD-dependent pyroptotic induction by a multivalent CXCR4-targeted nanotoxin blocks colorectal cancer metastases. Drug Delivery, 29, 1384-1397. https://doi.org/10.1080/10717544.2022.2069302

Esperanza Medina-Gutiérrez, Annabel García-León, Alberto Gallardo, Patricia Álamo, Lorena Alba-Castellón, Ugutz Unzueta, Antonio Villaverde, Esther Vazquez, Isolda Casanova, Ramon Mangues. 2022. Potent anticancer activity of CXCR4-targeted nanostructured toxins in aggressive endometrial cancer models. Cancers, 15; 85. https://doi.org/10.3390/cancers15010085

Nanoconjugate

María Virtudes Céspedes, Ugutz Unzueta. Anna Avinyó, Alberto Gallardo, Patricia Alamo, Rita Sala, Alejandro Sanchez-Chardi, Isolda Casanova, María Antonia Mangues, Antonio Lopez-Pousa, Ramón Eritja, Antonio Villaverde, Esther Vazquez, Ramon Mangues. 2018. Selective depletion of metastatic stem cells as therapy for human colorectal cancer. EMBO Molecular Medicine, e8772. https://doi.org/10.15252/emmm.201708772