Publications

Nanoligent prides itself on contributing to the scientific community through extensive research publications. Our work, published in prestigious journals, reflects groundbreaking advancements in nanobiotechnology and cancer therapy.

The insights shared by our experts not only pave the way for new treatment approaches but also inspire collaborative efforts worldwide. Discover our published findings that are shaping the future of precision medicine and offering new hope in the fight against cancer.

Mechanism of action:

Ugutz Unzueta, María Virtudes Céspedes, Neus Ferrer-Miralles, Isolda Casanova, Juan Cedano, José Luis Corchero, Joan Domingo-Espín, Antonio Villaverde, Ramón Mangues, Esther Vázquez. 2012. Intracellular CXCR4+ cell targeting with T22-empowered protein-only nanoparticles. International Journal of Nanomedicine 7: 4533 – 4544.
https://doi.org/10.2147/IJN.S34450

Laura Sánchez-García, Naroa Serna, Patricia Álamo, Rita Sala, María V Céspedes, Mònica Roldan, Alejandro Sánchez-Chardi, Ugutz Unzueta, Isolda Casanova, Ramon Mangues, Esther Vázquez, Antonio Villaverde. 2018. Self-assembling toxin-based nanoparticles as self-delivered antitumoral drugs. Journal of Controlled Release, 274: 81-92.
https://doi.org/10.1016/j.jconrel.2018.01.031

Aïda Falgàs, Victor Pallarès, Ugutz Unzueta, María Virtudes Céspedes, Irene Arroyo-Solera, María José Moreno, Alberto Gallardo, María Antonia Mangues, Jorge Sierra, Antonio Villaverde, Esther Vázquez, Ramon Mangues, and Isolda Casanova. 2020. A CXCR4-targeted nanocarrier achieves highly selective tumor uptake in diffuse large B-cell lymphoma mouse models. Haematologica. 2020. 105: 741-753.
https://doi.org/10.3324/haematol.2018.211490

Vehicle design and properties:

Céspedes MV, Unzueta U, Tatkiewicz W, Sánchez-Chardi A, Conchillo-Solé O, Alamo P, Xu Z, Casanova I, Corchero JL, Pesarrodona M, Cedano J, Daura X, Ratera I, Veciana J, Ferrer-Miralles N, Vazquez E, Villaverde A, Mangues R. 2014. In Vivo Architectonic Stability of Fully de Novo Designed Protein-Only Nanoparticles. ACS Nano. 8:4166-4176.
https://doi.org/10.1021/nn4055732

Unzueta U, Céspedes MV, Ferrer-Miralles N, Vazquez E, Mangues R., Villaverde A. 2015. Towards Protein-Based Viral Mimetics for Cancer Therapies. Trends in Biotechnology, 33: 253-258.
https://doi.org/10.1016/j.tibtech.2015.02.007

Naroa Serna, Laura Sánchez-García, Ugutz Unzueta, Raquel Díaz, Esther Vázquez, Ramón Mangues and Antonio Villaverde. 2018. Protein-based therapeutic killing for cancer therapies. Trends in Biotechnology, 36: 318-335.
https://doi.org/10.1016/j.tibtech.2017.11.007

Hèctor López-Laguna, Julieta Sánchez, Ugutz Unzueta, Ramón Mangues, Esther Vázquez and Antonio Villaverde. 2020. Divalent cations: A molecular glue for protein materials. Trends in Biochemical Sciences 45: 992-1003.
https://doi.org/10.1016/j.tibs.2020.08.003

Patricia Alamo, Juan Cedano, Oscar Conchillo-Sole, Olivia Cano-Garrido, “Lorena Alba-Castellon, Naroa Serna, Anna Aviño, Luis Miguel Carrasco-Diaz, Alejandro Sanchez-chardi, Carlos Martinez-Torro, Alberto Gallardo, Montserrat Cano, Ramon Eritja, Antonio Villaverde, Ramon Mangues, Esther Vazquez, Ugutz Unzueta. 2021. Rational engineering of a human GFP-like protein scaffold for humanized targeted nanomedicines. Acta Biomaterialia 130, 211-222.
https://doi.org/10.1016/j.actbio.2021.06.001

López-Laguna, Hèctor; Sánchez, Julieta; Carratalá, José Vicente; Rojas-Peña, Mauricio; Sánchez-García, Laura; Parladé, Eloi; Sánchez-Chardi, Alejandro; Voltà-Durán, Eric; Serna, Naroa; Cano-Garrido, Olivia; Flores, Sandra; Ferrer-Miralles, Neus; Nolan, Verónica; de Marco, Ario; Roher, Nerea; Unzueta, Ugutz; Vazquez, Esther; Villaverde, Antonio. 2021. Biofabrication of functional protein nanoparticles through simple His-tag engineering. ACS Sustainable Chemistry & Engineering, 9, 12341-12354.
https://doi.org/10.1021/acssuschemeng.1c04256

Naroa Serna, Victor Pallarès, Ugutz Unzueta, Alejandro Sánchez-Chardi, Eric Voltà-Durán, Eloi Parladé, Isolda Casanova, Aïda Falgàs, Lorena Alba-Castellón, Jorge Sierra, Antonio Villaverde, Esther Vázquez and Ramón Mangues. 2022. Engineering non-antibody human proteins as efficient scaffolds for selective, receptor-targeted drug delivery. Journal of Controlled Release, 343: 277-287.
https://doi.org/10.1016/j.jconrel.2022.01.017

Efficacy on liquid tumors:

• Nanotoxin

Aïda Falgàs, Victor Pallarès, Naroa Serna, Laura Sánchez-García, Jorge Sierra, Alberto Gallardo, Lorena Alba-Castellón, Patricia Álamo, Ugutz Unzueta, Antonio Villaverde, Esther Vázquez, Ramon Mangues and Isolda Casanova. 2020. Selective delivery of T22-PE24-H6 to CXCR4+ diffuse large B-cell lymphoma cells leads to wide therapeutic index in a disseminated mouse model. Theranostics. 10: 5169-5180.
https://www.thno.org/v10p5169.htm

Victor Pallarès, Yáiza Núñez, Laura Sánchez-García, Aïda Falgàs, Naroa Serna, Ugutz Unzueta, Alberto Gallardo, Lorena Alba-Castellón, Patricia Álamo, Jorge Sierra, Antonio Villaverde, Esther Vázquez, Isolda Casanova, Ramon Mangues. 2021. Antineoplastic effect of a diphtheria toxin-based nanoparticle targeting acute myeloid leukemia cells overexpressing CXCR4. Journal of Controlled Release 335: 117-129.
https://doi.org/10.1016/j.jconrel.2021.05.014

Yáiza Núñez, Annabel Garcia-León, Aïda Falgàs, Naroa Serna, Laura Sánchez-García, Ana Garrido, Jorge Sierra, Alberto Gallardo, Lorena Alba-Castellón, Patricia Álamo, Ugutz Unzueta, Esther Vázquez, Antonio Villaverde, Ramon Mangues, Isolda Casanova. 2023. T22-PE24-H6 nanotoxin selectively kills CXCR4-high expressing AML patient cells in vitro and potently blocks dissemination in vivo. Pharmaceutics 15, 727.
https://doi.org/10.3390/pharmaceutics15030727

• Nanoconjugate

Naroa Serna; Victor Pallarès; Ugutz Unzueta; Aïda Falgàs; Laura Sánchez-García; Naroa Serna; Alberto Gallardo; Gordon A. Morris; Lorena Alba-Castellón; Patricia Álamo; Jorge Sierra; Antonio Villaverde; Esther Vázquez; Isolda Casanova; Ramon Mangues. 2020. An Auristatin nanoconjugate targeting CXCR4+ leukemic cells blocks acute myeloid leukemia dissemination. Journal of Hematology & Oncology, 13, 1-19.
https://doi.org/10.1186/s13045-020-00863-9

Aïda Falgàs, Victor Pallarès, Ugutz Unzueta, Yáiza Núñez, Annabel García-León, Jorge Sierra, Alberto Gallardo, Lorena Alba-Castellón, Maria Antònia Mangues, Patricia Álamo, Antonio Villaverde, Esther Vázquez, Ramon Mangues and Isolda Casanova. 2021. Specific cytotoxic effect of an auristatin nanoconjugate towards CXCR4+ diffuse large B-cell lymphoma cells. International Journal of Nanomedicine 16:1869-1888.
https://doi.org/10.2147/IJN.S289733

Victor Pallarès, Ugutz Unzueta, Aïda Falgàs, Anna Aviñó, Yáiza Núñez, Annabel García-León, Laura Sánchez-García, Naroa Serna, Alberto Gallardo, Lorena Alba-Castellón, Patricia Álamo, Jorge Sierra, Lídia Cedó, Ramón Eritja, Antonio Villaverde, Esther Vázquez, Isolda Casanova, Ramon Mangues. 2022. A multivalent Ara-C-prodrug nanoconjugate achieves selective ablation of leukemic cells in an acute myeloid leukemia mouse model. Biomaterials 280, 121258.
https://doi.org/10.1016/j.biomaterials.2021.121258

Efficacy on solid tumors:

• Nanoconjugate

Raquel Díaz, Victor Pallarès, Olivia Cano-Garrido, Naroa Serna, Laura Sánchez-Garcia, Aïda Falgàs, Mireia Pesarrodona, Ugutz Unzueta, Alejandro Sánchez-Chardi, Julieta M. Sánchez, Isolda Casanova, Dr Esther Vázquez, Ramón Mangues, Antonio Villaverde. 2018. Selective CXCR4+ cancer cell targeting and potent antineoplastic effect by a nanostructured version of recombinant ricin. Small, 14: 1800665.
https://doi.org/10.1002/smll.201800665

Naroa Serna, Patricia Álamo, Prashanthi Ramesh, Daria Vinokurova, Laura Sánchez-García, Ugutz Unzueta, Alberto Gallardo, Mª Virtudes Céspedes, Esther Vázquez, Antonio Villaverde, Ramón Mangues and Jan Paul Medema. 2020. Nanostructured Toxins for the Selective Destruction of Drug Resistant Human CXCR4+ Colorectal Cancer Stem Cells. Journal of Controlled Release 320: 96–104.
https://doi.org/10.1016/j.jconrel.2020.01.019

Elisa Rioja-Blanco; Irene Arroyo-Solera; Patricia Álamo; Isolda Casanova; Alberto Gallardo; Ugutz Unzueta; Naroa Serna; Laura Sánchez-García; Miquel Quer; Antonio Villaverde; Esther Vázquez; Ramon Mangues; Lorena Alba-Castellón; Xavier León. 2022. Self-assembling protein nanocarrier for the selective delivery of cytotoxic polypeptides to CXCR4+ head and neck squamous cell carcinoma tumors. Acta Pharmaceutica Sinica B, 12: 2578-2591.
https://doi.org/10.1016/j.apsb.2021.09.030

Elisa Rioja-Blanco, Irene Arroyo-Solera, Patricia Álamo, Isolda Casanova, Alberto Gallardo, Ugutz Unzueta, Naroa Serna, Laura Sánchez-García, Miquel Quer, Antonio Villaverde, Esther Vázquez, Xavier León, Lorena Alba-Castellón & Ramon Mangues. 2022. CXCR4-targeted nanotoxins induce GSDME-dependent pyroptosis in head and neck squamous cell carcinoma. Journal of Experimental & Clinical Cancer Research, 41: 49.
https://doi.org/10.1186/s13046-022-02267-8

Aïda Falgàs, Annabel Garcia-León, Yáiza Núñez, Naroa Serna, Laura Sánchez-Garcia, Ugutz Unzueta, EricVoltà-Durán, Marc Aragó, Patricia Álamo, Lorena Alba-Castellón, JorgeSierra, Alberto Gallardo, Antonio Villaverde, Esther Vázquez, Ramon Mangues, Isolda Casanova. 2022. A diphtheria toxin-based nanoparticle achieves specific cytotoxic effect on CXCR4+ lymphoma cells without toxicity in immunocompromised and immunocompetent mice. Biomedicine & Pharmacotherapy, 150: 112940.
https://doi.org/10.1016/j.biopha.2022.112940

Elisa Rioja-Blanco, Alberto Gallardo, Irene Arroyo-Solera, Patricia Álamo, Isolda Casanova, Ugutz Unzueta, Naroa Serna, Laura Sánchez-García, Miquel Quer, Antonio Villaverde, Esther Vázquez, Xavier León, Lorena Alba-Castellón, Ramon Mangues. 2022. A novel CXCR4-targeted diphtheria toxin nanoparticle inhibits invasion and metastatic dissemination in a head and neck squamous cell carcinoma mouse model. Pharmaceutics, 14, 887.
https://doi.org/10.3390/pharmaceutics14040887

Rita Sala, Elisa Rioja-Blanco, Naroa Serna, Laura Sánchez-García, Patricia Álamo, Lorena Alba-Castellón, Isolda Casanova, Antonio López-Pousa, Ugutz Unzueta, María Virtudes Céspedes, Esther Vázquez, Antonio Villaverde & Ramon Mangues. 2022. GSDMD-dependent pyroptotic induction by a multivalent CXCR4-targeted nanotoxin blocks colorectal cancer metastases. Drug Delivery, 29, 1384-1397.
https://doi.org/10.1080/10717544.2022.2069302

Esperanza Medina-Gutiérrez, Annabel García-León, Alberto Gallardo, Patricia Álamo, Lorena Alba-Castellón, Ugutz Unzueta, Antonio Villaverde, Esther Vazquez, Isolda Casanova, Ramon Mangues. 2022. Potent anticancer activity of CXCR4-targeted nanostructured toxins in aggressive endometrial cancer models. Cancers, 15; 85.
https://doi.org/10.3390/cancers15010085

• Nanotoxin

María Virtudes Céspedes, Ugutz Unzueta. Anna Avinyó, Alberto Gallardo, Patricia Alamo, Rita Sala, Alejandro Sanchez-Chardi, Isolda Casanova, María Antonia Mangues, Antonio Lopez-Pousa, Ramón Eritja, Antonio Villaverde, Esther Vazquez, Ramon Mangues. 2018. Selective depletion of metastatic stem cells as therapy for human colorectal cancer. Selective depletion of metastatic stem cells as therapy for human colorectal cancer.
https://doi.org/10.15252/emmm.201708772